Can yesterday’s intraday profits be used for today’s trade in Zerodha?

Kite balance will not include any intraday profits if they are not settled by the exchange. The settlement of funds will happen on the next trading day (T+1) for all equity and F&O trades. This is due to SEBI’s new upfront margin requirements. To learn more, see What changes due to the new upfront margin requirements?

Example scenario

1. Shares worth ₹2,00,000 are bought on Monday.
2. These shares are sold on the same day for ₹ 2,25,000 with a profit of ₹25,000 (ignoring all the charges).
3. ₹2,00,000 will be available immediately for other transactions. However, the profit of ₹25,000 will be available only on Tuesday for withdrawal or to be used for other trades based on the T+1 settlement cycle.

If there is a settlement holiday, it will take an additional day for the settlement process. See What is a settlement holiday and its impact?, Market holiday calendar 2023.